GTF2E1 Antibody
Product Specifications
Background
The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation
NCBI Gene ID
2960
Swiss Prot
P29083
Accession Number
NP_005504
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1224 - Daudi Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GTF2E1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=31-216
Calculated Molecular Weight
49 kDa
Applications Notes
GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:1562500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 0.2 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
GTF2E1
NCBI Official Name
General transcription factor IIE, polypeptide 1, alpha 56kDa
NCBI Organism
Homo sapiens
Background Reference 01
Ohkuma, Y., et al., (1991) Nature 354 (6352), 398-401.
Other Product Names
GTF2E1, FE, TF2E1, TFIIE-A
Tested Applications
ELISA, WB, IHC
Protein ID
144922725
Physical Properties
Liquid
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