SRF Antibody

SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs) .

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SRF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

SRF antibody can be used for detection of SRF by ELISA at 1:1562500. SRF antibody can be used for detection of SRF by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.