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NR2F2 Antibody

Product Specifications

Background

NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.

NCBI Gene ID

7026

Swiss Prot

P24468

Accession Number

NP_066285

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR2F2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer, Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1219 - MCF7 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NR2F2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=31-192

Calculated Molecular Weight

45 kDa

Applications Notes

NR2F2 antibody can be used for detection of NR2F2 by ELISA at 1:62500. NR2F2 antibody can be used for detection of NR2F2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

NR2F2

NCBI Official Name

Nuclear receptor subfamily 2, group F, member 2

NCBI Organism

Homo sapiens

Background Reference 01

Schafer, G., (2008) Cancer Res. 68 (2), 457-466.

Other Product Names

NR2F2, ARP1, COUP-TFII, COUPTFB, MGC117452, SVP40, TFCOUP2, NF-E3, NR2F1, COUPTFII

Tested Applications

ELISA, WB

Protein ID

14149746

Physical Properties

Liquid

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