NR2C1 Antibody

Product Specifications

Background

The nuclear orphan receptors NR2C1 represses transcription and binds DNA as a homodimer. NR2C1 binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. NR2C1 may function as a negative modulator to suppress androgen receptor function in prostate cancer. NR2C1 may exert an important repressor in regulating ER activity in mammary glands. The nuclear orphan receptors TR2 (NR2C1) and TR4 form a heterodimer that binds to the epsilon and gamma globin promoter DR1 sites

NCBI Gene ID

7181

Swiss Prot

Q15625

Accession Number

NP_003288

Host

Rabbit

Reactivity

Human, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR2C1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

HCT15 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NR2C1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

67 kDa

Applications Notes

NR2C1 antibody can be used for detection of NR2C1 by ELISA at 1:312500. NR2C1 antibody can be used for detection of NR2C1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.