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TCF4 Antibody

Product Specifications

Background

TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. TCF4 is known to produce multiple transcripts; however as the complete structure is only known for the transcript that encodes the b isoform, that is the variant presented here.

NCBI Gene ID

6925

Swiss Prot

P15884

Accession Number

NP_003190

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF4.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TCF4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

71 kDa

Applications Notes

TCF4 antibody can be used for detection of TCF4 by ELISA at 1:62500. TCF4 antibody can be used for detection of TCF4 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

TCF4

NCBI Official Name

Transcription factor 4

NCBI Organism

Homo sapiens

Background Reference 01

Raurell, I., (2006) J. Biol. Chem. 281 (3), 1401-1411.

Other Product Names

TCF4, E2-2, ITF2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, PTHS, ITF-2, SEF-2, TCF-4, SEF2-1D, bHLHb19

Tested Applications

ELISA, WB

Protein ID

4507399

Physical Properties

Liquid

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