CHRNA3 Antibody
Product Specifications
Background
The CHRNA3 subunit is expressed in the soma of the majority of pyramidal cells, with the most alpha 3 immunoreactivity observed in CA2-4 and entorhinal cortex and relatively less in CA1 and subicular pyramidal cell soma.
NCBI Gene ID
1136
Swiss Prot
Q86U77
Accession Number
NP_000734
Host
Rabbit
Reactivity
Human, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNA3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Membrane, Cancer, Signal Transduction
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CHRNA3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
57 kDa
Applications Notes
CHRNA3 antibody can be used for detection of CHRNA3 by ELISA at 1:1562500. CHRNA3 antibody can be used for detection of CHRNA3 by western blot at 0.625 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
CHRNA3
NCBI Official Name
Cholinergic receptor, nicotinic, alpha 3 (neuronal)
NCBI Organism
Homo sapiens
Background Reference 01
Arredondo, J., et al., (2005) Am. J. Pathol. 166 (2), 597-613.
Other Product Names
CHRNA3, LNCR2, PAOD2, NACHRA3
Tested Applications
ELISA, WB
Protein ID
19923122
Physical Properties
Liquid
Curated Selection
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