CCNH Antibody

Cyclin H regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive carboxyl-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. It is involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CCNH antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

CCNH antibody can be used for detection of CCNH by ELISA at 1:75000. CCNH antibody can be used for detection of CCNH by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.