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DIABLO Antibody

Product Specifications

Background

DIABLO is an inhibitor of apoptosis protein (IAP) -binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively.

NCBI Gene ID

56616

Swiss Prot

Q502X2

Accession Number

NP_620308

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DIABLO.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DIABLO antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

21 kDa, 27 kDa, 22 kDa

Applications Notes

DIABLO antibody can be used for detection of DIABLO by ELISA at 1:312500. DIABLO antibody can be used for detection of DIABLO by western blot at 0.3125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

DIABLO

NCBI Official Name

Diablo, IAP-binding mitochondrial protein

NCBI Organism

Homo sapiens

Background Reference 01

Samuel, T., et al., (2006) J. Biol. Chem. 281 (2), 1080-1090.

Other Product Names

DIABLO, SMAC, DFNA64

Tested Applications

ELISA, WB

Protein ID

21070978

Physical Properties

Liquid

Frequently Asked Questions

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