CARD12 Antibody
Product Specifications
Background
In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs) . Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs) . CARD12 is a member of the Ced4 family and can induce apoptosis.
NCBI Gene ID
58484
Swiss Prot
Q9NPP4
Accession Number
NP_067032
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CARD12.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Apoptosis
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CARD12 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=30-999
Calculated Molecular Weight
116 kDa
Applications Notes
CARD12 antibody can be used for detection of CARD12 by ELISA at 1:62500. CARD12 antibody can be used for detection of CARD12 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
NLRC4
NCBI Official Name
NLR family, CARD domain containing 4
NCBI Organism
Homo sapiens
Background Reference 01
Clark, H.F., et al., (2003) Genome Res. 13 (10), 2265-2270.
Other Product Names
CARD12, CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1
Tested Applications
ELISA, WB, IHC
Protein ID
40788015
Physical Properties
Liquid
Curated Selection
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