ATG10 Antibody
Product Specifications
Background
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form.Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608) -ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]) .
NCBI Gene ID
83734
Swiss Prot
Q9H0Y0
Accession Number
NP_113670
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATG10.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Autophagy
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATG10 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
25 kDa
Applications Notes
ATG10 antibody can be used for detection of ATG10 by ELISA at 1:312500. ATG10 antibody can be used for detection of ATG10 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ATG10
NCBI Official Name
Autophagy related 10
NCBI Organism
Homo sapiens
Background Reference 01
Criollo, A., (2007) Cell Death Differ. 14 (5), 1029-1039.
Other Product Names
ATG10, APG10, APG10L, DKFZP586I0418, FLJ13954, pp12616
Tested Applications
ELISA, WB
Protein ID
18594496
Physical Properties
Liquid
Curated Selection
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