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ATP5B Antibody

Product Specifications

Background

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

NCBI Gene ID

506

Swiss Prot

P06576

Accession Number

NP_001677

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP5B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATP5B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=30-375

Calculated Molecular Weight

52 kDa

Applications Notes

ATP5B antibody can be used for detection of ATP5B by ELISA at 1:312500. ATP5B antibody can be used for detection of ATP5B by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

ATP5B

NCBI Official Name

ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide

NCBI Organism

Homo sapiens

Background Reference 01

Vantourout, P., (2008) Mol. Immunol. 45 (2), 485-492.

Other Product Names

ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271

Tested Applications

ELISA, WB

Protein ID

32189394

Physical Properties

Liquid

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