ATP5B Antibody
Product Specifications
Background
ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
NCBI Gene ID
506
Swiss Prot
P06576
Accession Number
NP_001677
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP5B.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATP5B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
52 kDa
Applications Notes
ATP5B antibody can be used for detection of ATP5B by ELISA at 1:312500. ATP5B antibody can be used for detection of ATP5B by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ATP5B
NCBI Official Name
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
NCBI Organism
Homo sapiens
Background Reference 01
Vantourout, P., (2008) Mol. Immunol. 45 (2), 485-492.
Other Product Names
ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271
Tested Applications
ELISA, WB
Protein ID
32189394
Physical Properties
Liquid
Curated Selection
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