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PGK1 Antibody

Product Specifications

Background

PGK1 is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The protein may also act as a cofactor for polymerase alpha.The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. A pseudogene of this gene has been found on the X-chromosome and another on chromosome 19. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

NCBI Gene ID

5230

Swiss Prot

P00558

Accession Number

NP_000282

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PGK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PGK1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

44 kDa

Applications Notes

PGK1 antibody can be used for detection of PGK1 by ELISA at 1:1562500. PGK1 antibody can be used for detection of PGK1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

PGK1

NCBI Official Name

Phosphoglycerate kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Gondeau, C., (2008) Biochemistry 47 (11), 3462-3473.

Other Product Names

PGK1, MGC117307, MGC142128, MGC8947, MIG10, PGKA, HEL-S-68p

Tested Applications

ELISA, WB, IHC

Protein ID

4505763

Physical Properties

Liquid

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