A4GNT Antibody
Product Specifications
Background
A4GNT is a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
NCBI Gene ID
51146
Swiss Prot
Q9UNA3
Accession Number
NP_057245
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human A4GNT.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Membrane, Cancer, Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store A4GNT antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
39 kDa
Applications Notes
A4GNT antibody can be used for detection of A4GNT by ELISA at 1:62500. A4GNT antibody can be used for detection of A4GNT by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
A4GNT
NCBI Official Name
Alpha-1,4-N-acetylglucosaminyltransferase
NCBI Organism
Homo sapiens
Background Reference 01
Ishizone, S., (2006) Cancer Sci. 97 (2), 119-126.
Other Product Names
A4GNT, MGC149493, alpha4GnT
Tested Applications
ELISA, WB
Protein ID
7705859
Physical Properties
Liquid
Curated Selection
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