DDOST Antibody

Product Specifications

Background

DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

NCBI Gene ID

1650

Swiss Prot

Q5VWA5

Accession Number

NP_005207

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDOST.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DDOST antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

46 kDa

Applications Notes

DDOST antibody can be used for detection of DDOST by ELISA at 1:62500. DDOST antibody can be used for detection of DDOST by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.