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EIF2S3 Antibody

Product Specifications

Background

EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

NCBI Gene ID

1968

Swiss Prot

P41091

Accession Number

NP_001406

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF2S3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

51 kDa

Applications Notes

EIF2S3 antibody can be used for detection of EIF2S3 by ELISA at 1:312500. EIF2S3 antibody can be used for detection of EIF2S3 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

EIF2S3

NCBI Official Name

Eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa

NCBI Organism

Homo sapiens

Background Reference 01

Gevaert, K., (2003) Nat. Biotechnol. 21 (5), 566-569.

Other Product Names

EIF2S3, EIF2, EIF2G, EIF2gamma, eIF-2gA

Tested Applications

ELISA, WB

Protein ID

4503507

Physical Properties

Liquid

Frequently Asked Questions

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