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UBE2I Antibody

Product Specifications

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family.

NCBI Gene ID

7329

Swiss Prot

P63279

Accession Number

NP_003336

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2I.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Apoptosis, Homeostasis

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store UBE2I antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

52 kDa, 51 kDa

Applications Notes

UBE2I antibody can be used for detection of UBE2I by ELISA at 1:1562500. UBE2I antibody can be used for detection of UBE2I by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

UBE2I

NCBI Official Name

Ubiquitin-conjugating enzyme E2I

NCBI Organism

Homo sapiens

Background Reference 01

Ota, T., (2004) Nat. Genet. 36 (1), 40-45.

Other Product Names

UBE2I, C358B7.1, P18, UBC9

Tested Applications

ELISA, WB

Protein ID

4507785

Physical Properties

Liquid

Frequently Asked Questions

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