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DHODH Antibody

Product Specifications

Background

DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

1723

Swiss Prot

Q02127

Accession Number

NP_001352

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DHODH.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DHODH antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

43 kDa

Applications Notes

DHODH antibody can be used for detection of DHODH by ELISA at 1:62500. DHODH antibody can be used for detection of DHODH by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

DHODH

NCBI Official Name

Dihydroorotate dehydrogenase (quinone)

NCBI Organism

Homo sapiens

Background Reference 01

Kimura, K., (2006) Genome Res. 16 (1), 55-65.

Other Product Names

DHODH, DHOdehase, URA1, POADS

Tested Applications

ELISA, WB

Protein ID

45006951

Physical Properties

Liquid

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