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SF1 Antibody

Product Specifications

Background

SF1 contains 1 KH domain and 1 CCHC-type zinc finger. It is necessary for the ATP-dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. It may act as transcription repressor.

NCBI Gene ID

7536

Swiss Prot

Q15637

Accession Number

NP_004621

Host

Rabbit

Reactivity

Human, Mouse, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

68 kDa, 69 kDa, 9 kDa, 60 kDa

Applications Notes

SF1 antibody can be used for detection of SF1 by ELISA at 1:312500. SF1 antibody can be used for detection of SF1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

SF1

NCBI Official Name

Splicing factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Huang, Y.H., (2006) Endocrinology 147 (5), 2481-2489.

Other Product Names

SF1, BBP, MBBP, ZFM1, ZNF162, D11S636, ZCCHC25

Tested Applications

ELISA, WB, IHC

Protein ID

42544130

Physical Properties

Liquid

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