SF3B14 Antibody

Product Specifications

Background

SF3B14 is a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. SF3B14 also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.

NCBI Gene ID

51639

Swiss Prot

Q7RTV0

Accession Number

NP_057131

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B14.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1309 - Human Placenta Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF3B14 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

14 kDa

Applications Notes

SF3B14 antibody can be used for detection of SF3B14 by ELISA at 1:62500. SF3B14 antibody can be used for detection of SF3B14 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.