SFPQ Antibody

Product Specifications

Background

SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

NCBI Gene ID

6421

Swiss Prot

P23246

Accession Number

NP_005057

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SFPQ.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SFPQ antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

78 kDa

Applications Notes

SFPQ antibody can be used for detection of SFPQ by ELISA at 1:12500. SFPQ antibody can be used for detection of SFPQ by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.