RPN1 Antibody
Product Specifications
Background
RPN1 is a type I integral membrane protein found only in the rough endoplasmic reticulum. It is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. RPN1 forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
NCBI Gene ID
6184
Swiss Prot
P04843
Accession Number
NP_002941
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RPN1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Membrane, Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1219 - MCF7 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RPN1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
66 kDa
Applications Notes
RPN1 antibody can be used for detection of RPN1 by ELISA at 1:62500. RPN1 antibody can be used for detection of RPN1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
RPN1
NCBI Official Name
Ribophorin I
NCBI Organism
Homo sapiens
Background Reference 01
Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007) .
Other Product Names
RPN1, DKFZp686B16177, OST1, RBPH1
Tested Applications
ELISA, WB
Protein ID
4506675
Physical Properties
Liquid
Curated Selection
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