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RPN1 Antibody

Product Specifications

Background

RPN1 is a type I integral membrane protein found only in the rough endoplasmic reticulum. It is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. RPN1 forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

NCBI Gene ID

6184

Swiss Prot

P04843

Accession Number

NP_002941

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RPN1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1219 - MCF7 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RPN1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

66 kDa

Applications Notes

RPN1 antibody can be used for detection of RPN1 by ELISA at 1:62500. RPN1 antibody can be used for detection of RPN1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

RPN1

NCBI Official Name

Ribophorin I

NCBI Organism

Homo sapiens

Background Reference 01

Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007) .

Other Product Names

RPN1, DKFZp686B16177, OST1, RBPH1

Tested Applications

ELISA, WB

Protein ID

4506675

Physical Properties

Liquid

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