PIGF Antibody

PIGF is a protein involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. PIGF and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. This gene encodes a protein involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PIGF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

PIGF antibody can be used for detection of PIGF by ELISA at 1:12500. PIGF antibody can be used for detection of PIGF by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.