EIF4B Antibody
Product Specifications
Background
IF4B contains 1 RRM (RNA recognition motif) domain and is required for the binding of mRNA to ribosomes. Functions of EIF4B are in close association with EEF4-F and EIF4-A. It binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP and promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
NCBI Gene ID
1975
Swiss Prot
P23588
Accession Number
NP_001408
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4B.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF4B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=29-271
Calculated Molecular Weight
67 kDa
Applications Notes
EIF4B antibody can be used for detection of EIF4B by ELISA at 1:62500. EIF4B antibody can be used for detection of EIF4B by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
EIF4B
NCBI Official Name
Eukaryotic translation initiation factor 4B
NCBI Organism
Homo sapiens
Background Reference 01
Suzuki, Y., (2004) Genome Res. 14 (9), 1711-1718.
Other Product Names
EIF4B, EIF-4B, PRO1843
Tested Applications
ELISA, WB, IHC
Protein ID
50053795
Physical Properties
Liquid
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