SF3B1 Antibody

Product Specifications

Background

SF3B1 is the subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome.

NCBI Gene ID

23451

Swiss Prot

O75533

Accession Number

AAH56155

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3B1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF3B1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

87 kDa, 16 kDa, 146 kDa

Applications Notes

SF3B1 antibody can be used for detection of SF3B1 by ELISA at 1:62500. SF3B1 antibody can be used for detection of SF3B1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.