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NR2F2 Antibody

Product Specifications

Background

NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.

NCBI Gene ID

7026

Swiss Prot

P24468

Accession Number

NP_066285

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR2F2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NR2F2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=29-063

Calculated Molecular Weight

46 kDa

Applications Notes

NR2F2 antibody can be used for detection of NR2F2 by ELISA at 1:1562500. NR2F2 antibody can be used for detection of NR2F2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

NR2F2

NCBI Official Name

Nuclear receptor subfamily 2, group F, member 2

NCBI Organism

Homo sapiens

Background Reference 01

Sato, Y., et al., (2003) J. Clin. Endocrinol. Metab. 88 (7), 3415-3420.

Other Product Names

NR2F2, ARP1, NF-E3, NR2F1, SVP40, COUPTFB, TFCOUP2, COUPTFII

Tested Applications

ELISA, WB

Protein ID

14149746

Physical Properties

Liquid

Curated Selection

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