GATAD2B Antibody

Product Specifications

Background

Hp66beta (GATAD2B), previously identified as a component of the human MeCP1 complex, is a second member of a p66 gene family (hp66alpha and hp66beta) . Both proteins interact with MBD2 and MBD3 functionally and biochemically. Both factors function in synergy, since knocking-down p66alpha affects the repressive function of p66beta and vice versa. Furthermore, confocal microscopy shows a nuclear colocalization of hp66alpha with hp66beta and MBD2 in a speckled pattern. These two highly related mammalian p66 proteins display overlapping functions and are involved in methylation dependent transcriptional repression.

NCBI Gene ID

57459

Swiss Prot

Q8WXI9

Accession Number

NP_065750

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATAD2B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GATAD2B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

65 kDa

Applications Notes

GATAD2B antibody can be used for detection of GATAD2B by ELISA at 1:312500. GATAD2B antibody can be used for detection of GATAD2B by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.