NFYB Antibody

NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFYB antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

NFYB antibody can be used for detection of NFYB by ELISA at 1:312500. NFYB antibody can be used for detection of NFYB by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.