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ATF1 Antibody

Product Specifications

Background

ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.

NCBI Gene ID

466

Swiss Prot

P18846

Accession Number

NP_005162

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1309 - Human Placenta Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

29 kDa

Applications Notes

ATF1 antibody can be used for detection of ATF1 by ELISA at 1:12500. ATF1 antibody can be used for detection of ATF1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

ATF1

NCBI Official Name

Activating transcription factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Iwasaki, K., (2007) J. Biol. Chem. 282 (31), 22335-22343.

Other Product Names

ATF1, EWS-ATF1, FUS/ATF-1, TREB36

Tested Applications

ELISA, WB

Protein ID

4885073

Physical Properties

Liquid

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