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NR5A2 Antibody

Product Specifications

Background

NR5A2 binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. It may be responsable for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. It is a key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. It may also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.

NCBI Gene ID

2494

Swiss Prot

O00482

Accession Number

NP_995582

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR5A2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NR5A2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=28-855

Calculated Molecular Weight

61 kDa, 56 kDa

Applications Notes

NR5A2 antibody can be used for detection of NR5A2 by ELISA at 1:12500. NR5A2 antibody can be used for detection of NR5A2 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

NR5A2

NCBI Official Name

Nuclear receptor subfamily 5, group A, member 2

NCBI Organism

Homo sapiens

Background Reference 01

Lee, Y.K., (2006) J. Biol. Chem. 281 (12), 7850-7855.

Other Product Names

NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, hB1F, hB1F-2, LRH1

Tested Applications

ELISA, WB

Protein ID

45545405

Physical Properties

Liquid

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