ENO1 Antibody
Product Specifications
Background
ENO1 is one of three enolase isoenzymes found in mammals; its gene encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
NCBI Gene ID
2023
Swiss Prot
P06733
Accession Number
NP_001419
Host
Rabbit
Reactivity
Human, Zebrafish, C. elegans, Arabidopsis
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ENO1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
47 kDa
Applications Notes
ENO1 antibody can be used for detection of ENO1 by ELISA at 1:62500. ENO1 antibody can be used for detection of ENO1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ENO1
NCBI Official Name
Enolase 1, (alpha)
NCBI Organism
Homo sapiens
Background Reference 01
Ghosh, A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330.
Other Product Names
ENO1, NNE, PPH, MPB1, ENO1L1
Tested Applications
ELISA, WB
Protein ID
4503571
Physical Properties
Liquid
Curated Selection
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