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MITF Antibody

Product Specifications

Background

MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

NCBI Gene ID

4286

Swiss Prot

O75030-9

Accession Number

NP_000239

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MITF.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MITF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=28-763

Calculated Molecular Weight

47 kDa, 58 kDa, 46 kDa, 58 kDa, 56 kDa, 55 kDa

Applications Notes

MITF antibody can be used for detection of MITF by ELISA at 1:1562500. MITF antibody can be used for detection of MITF by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

MITF

NCBI Official Name

Microphthalmia-associated transcription factor

NCBI Organism

Homo sapiens

Background Reference 01

Park, H.Y., et al., (2006) Biochem. J. 395 (3), 571-578.

Other Product Names

MITF, MI, WS2, CMM8, WS2A, bHLHe32

Tested Applications

ELISA, WB

Protein ID

4557755

Physical Properties

Liquid

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