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SFPI1 Antibody

Product Specifications

Background

Sfpi1 binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Sfpi1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Sfpi1 also binds RNA and may modulate pre-mRNA splicing.

NCBI Gene ID

20375

Swiss Prot

P17433

Accession Number

NP_035485

Host

Rabbit

Reactivity

Mouse

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SFPI1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1369 - Human Lymph node Tissue Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SFPI1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=28-754

Calculated Molecular Weight

30 kDa

Applications Notes

SFPI1 antibody can be used for detection of SFPI1 by ELISA at 1:312500. SFPI1 antibody can be used for detection of SFPI1 by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

Spi1

NCBI Official Name

Spleen focus forming virus (SFFV) proviral integration oncogene

NCBI Organism

Mus musculus

Background Reference 01

McDevit, D.C. (2006) Mol. Immunol. 43 (10), 1541-1548.

Other Product Names

SFPI1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1

Tested Applications

ELISA, WB

Protein ID

6755474

Physical Properties

Liquid

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