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G3BP Antibody

Product Specifications

Background

G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

NCBI Gene ID

10146

Swiss Prot

Q13283

Accession Number

NP_005745

Host

Rabbit

Reactivity

Human, Mouse, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human G3BP.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Homeostasis

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store G3BP antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

51 kDa, 52 kDa

Applications Notes

G3BP antibody can be used for detection of G3BP by ELISA at 1:312500. G3BP antibody can be used for detection of G3BP by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

G3BP1

NCBI Official Name

GTPase activating protein (SH3 domain) binding protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Kociok, N., et al., (1999) J. Cell. Biochem. 74 (2), 194-201.

Other Product Names

G3BP, G3BP, HDH-VIII

Tested Applications

ELISA, WB

Protein ID

5031703

Physical Properties

Liquid

Frequently Asked Questions

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