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GPC3 Antibody

Product Specifications

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome.

NCBI Gene ID

2719

Swiss Prot

P51654

Accession Number

NP_004475

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPC3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Signal Transduction

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1309 - Human Placenta Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GPC3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

64 kDa

Applications Notes

GPC3 antibody can be used for detection of GPC3 by ELISA at 1:1562500. GPC3 antibody can be used for detection of GPC3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

GPC3

NCBI Official Name

Glypican 3

NCBI Organism

Homo sapiens

Background Reference 01

Boily, G., et al., (2004) Br. J. Cancer 90 (8), 1606-1611.

Other Product Names

GPC3, SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2

Tested Applications

ELISA, WB

Protein ID

4758462

Physical Properties

Liquid

Frequently Asked Questions

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