YAF2 Antibody

Product Specifications

Background

Yaf2 binds to MYC and inhibits MYC-mediated transactivation. Yaf2 also binds to MYCN and enhances MYCN-dependent transcriptional activation. Yaf2 increases calpain 2-mediated proteolysis of YY1 in vitro. Yaf2 is a component of the E2F6.com-1 complex, a repressive complex that methylates Lys-9 of histone H3, suggesting that it is involved in chromatin-remodeling.

NCBI Gene ID

67057

Swiss Prot

Q99LW6

Accession Number

NP_077151

Host

Rabbit

Reactivity

Mouse

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse YAF2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store YAF2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

20 kDa

Applications Notes

YAF2 antibody can be used for detection of YAF2 by ELISA at 1:312500. YAF2 antibody can be used for detection of YAF2 by western blot at 0.2 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.