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NKX2-2 Antibody

Product Specifications

Background

Nkx2-2 contains 1 homeobox DNA-binding domain which is essential for interaction with OLIG2. Nkx2-2 may be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.

NCBI Gene ID

18088

Swiss Prot

P42586

Accession Number

NP_035049

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse NKX2-2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

SP2/0 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NKX2-2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

30 kDa

Applications Notes

NKX2-2 antibody can be used for detection of NKX2-2 by ELISA at 1:1562500. NKX2-2 antibody can be used for detection of NKX2-2 by western blot at 0.125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

NKX2-2

NCBI Official Name

NK2 homeobox 2

NCBI Organism

Mus musculus

Background Reference 01

Sun, T., et al., (2006) Dev. Biol. 292 (1), 152-164.

Other Product Names

NKX2-2, Nkx2.2, tinman, Nkx-2.2

Tested Applications

ELISA, WB

Protein ID

6754860

Physical Properties

Liquid

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