FOXH1 Antibody

Product Specifications

Background

Foxh1 is a transcriptional activator. Foxh1 can recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3' and is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Foxh1 forms a transcriptionally active complex containing FAST-1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element) .

NCBI Gene ID

14106

Swiss Prot

O88621

Accession Number

NP_032015

Host

Rabbit

Reactivity

Mouse

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse FOXH1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store FOXH1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

44 kDa

Applications Notes

FOXH1 antibody can be used for detection of FOXH1 by ELISA at 1:62500. FOXH1 antibody can be used for detection of FOXH1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.