MCM8 Antibody

Product Specifications

Background

MCM8 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.

NCBI Gene ID

84515

Swiss Prot

Q9UJA3

Accession Number

NP_115874

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM8.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MCM8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

92 kDa, 92 kDa

Applications Notes

MCM8 antibody can be used for detection of MCM8 by ELISA at 1:62500. MCM8 antibody can be used for detection of MCM8 by western blot at 0.125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.