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DDX54 Antibody

Product Specifications

Background

DDX54 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein interacts in a hormone-dependent manner with nuclear receptors and represses their transcriptional activity.This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors and represses their transcriptional activity.

NCBI Gene ID

79039

Swiss Prot

Q8TDD1

Accession Number

NP_076977

Host

Rabbit

Reactivity

Human, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX54.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Signal Transduction, Immunology

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Tranfected 293T Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DDX54 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

97 kDa

Applications Notes

DDX54 antibody can be used for detection of DDX54 by ELISA at 1:312500. DDX54 antibody can be used for detection of DDX54 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

DDX54

NCBI Official Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 54

NCBI Organism

Homo sapiens

Background Reference 01

Rajendran, R.R., (2003) J. Biol. Chem. 278 (7), 4628-4638.

Other Product Names

DDX54, DP97, MGC2835

Tested Applications

ELISA, WB

Protein ID

51094101

Physical Properties

Liquid

Frequently Asked Questions

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