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HMGB1 Antibody

Product Specifications

Background

Extracellular HMGB1 is an activator of human tumor cell migration operating in concert with EGF. HMGB1 encodes a protein that is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription.

NCBI Gene ID

3146

Swiss Prot

Q5T7C3

Accession Number

NP_002119

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGB1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store HMGB1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

25 kDa, 24 kDa

Applications Notes

HMGB1 antibody can be used for detection of HMGB1 by ELISA at 1:62500. HMGB1 antibody can be used for detection of HMGB1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

HMGB1

NCBI Official Name

High mobility group box 1

NCBI Organism

Homo sapiens

Background Reference 01

Messmer, D., et al., (2004) J. Immunol. 173 (1), 307-313.

Other Product Names

HMGB1, HMG1, HMG3, SBP-1

Tested Applications

ELISA, WB

Protein ID

4504425

Physical Properties

Liquid

Frequently Asked Questions

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