CHRNA5 Antibody
Product Specifications
Background
Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero) pentamers composed of homologous subunits.
NCBI Gene ID
1138
Swiss Prot
P30532
Accession Number
NP_000736
Host
Rabbit
Reactivity
Human, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNA5.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Membrane, Cancer, Signal Transduction
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CHRNA5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
53 kDa
Applications Notes
CHRNA5 antibody can be used for detection of CHRNA5 by ELISA at 1:12500. CHRNA5 antibody can be used for detection of CHRNA5 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
CHRNA5
NCBI Official Name
Cholinergic receptor, nicotinic, alpha 5 (neuronal)
NCBI Organism
Homo sapiens
Background Reference 01
Groot-Kormelink, P.J., et al., (2001) Br. J. Pharmacol. 134 (4), 789-796.
Other Product Names
CHRNA5, LNCR2
Tested Applications
ELISA, WB
Protein ID
33589827
Physical Properties
Liquid
Curated Selection
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