TAF9 Antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF9 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

TAF9 antibody can be used for detection of TAF9 by ELISA at 1:312500. TAF9 antibody can be used for detection of TAF9 by western blot at 0.125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.