NFKBIB Antibody
Product Specifications
Background
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) .
NCBI Gene ID
4793
Swiss Prot
Q15653
Accession Number
NP_002494
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKBIB.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFKBIB antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=28-110
Calculated Molecular Weight
38 kDa, 33 kDa
Applications Notes
NFKBIB antibody can be used for detection of NFKBIB by ELISA at 1:312500. NFKBIB antibody can be used for detection of NFKBIB by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
NFKBIB
NCBI Official Name
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
NCBI Organism
Homo sapiens
Background Reference 01
Joo, M., (2005) J. Virol. 79 (12), 7648-7657.
Other Product Names
NFKBIB, IKBB, TRIP9
Tested Applications
ELISA, WB, IHC
Protein ID
21361135
Physical Properties
Liquid
Curated Selection
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