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RRN3 Antibody

Product Specifications

Background

In mammals, growth-dependent regulation of RNA polymerase I (Pol I) transcription is mediated by RRN3, an essential initiation factor. It interacts with Pol I in the absence of template DNA, augments Pol I transcription in vivo and rescues transcription in extracts from growth-arrested cells in vitro.

NCBI Gene ID

54700

Swiss Prot

Q9NYV6

Accession Number

NP_060897

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RRN3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RRN3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

74 kDa

Applications Notes

RRN3 antibody can be used for detection of RRN3 by ELISA at 1:62500. RRN3 antibody can be used for detection of RRN3 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

RRN3

NCBI Official Name

RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)

NCBI Organism

Homo sapiens

Background Reference 01

Miller, G., et al., (2001) EMBO J. 20 (6), 1373-1382.

Other Product Names

RRN3, TIFIA, A-270G1.2

Tested Applications

ELISA, WB

Protein ID

93102377

Physical Properties

Liquid

Frequently Asked Questions

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