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VPS24 Antibody

Product Specifications

Background

VPS24 encodes a protein that acts in the sorting of transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily.

NCBI Gene ID

51652

Swiss Prot

Q3ZTS9

Accession Number

NP_057163

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human VPS24.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store VPS24 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

25 kDa

Applications Notes

VPS24 antibody can be used for detection of VPS24 by ELISA at 1:12500. VPS24 antibody can be used for detection of VPS24 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

CHMP3

NCBI Official Name

Charged multivesicular body protein 3

NCBI Organism

Homo sapiens

Background Reference 01

Whitley, P., et al., (2003) J Biol Chem. 278 (40), 38786-95.

Other Product Names

VPS24, NEDF, VPS24, CGI-149

Tested Applications

ELISA, WB

Protein ID

7706353

Physical Properties

Liquid

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