XRCC5 Antibody

XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V (D) J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V (D) J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store XRCC5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

XRCC5 antibody can be used for detection of XRCC5 by ELISA at 1:12500. XRCC5 antibody can be used for detection of XRCC5 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.