PIR Antibody
Product Specifications
Background
PIR is a member of the cupin superfamily. The protein is an Fe (II) -containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication.This gene encodes a member of the cupin superfamily. The encoded protein is an Fe (II) -containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.
NCBI Gene ID
8544
Swiss Prot
O00625
Accession Number
NP_003653
Host
Rabbit
Reactivity
Human, Mouse, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIR.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PIR antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=27-915
Calculated Molecular Weight
32 kDa, 32 kDa
Applications Notes
PIR antibody can be used for detection of PIR by ELISA at 1:312500. PIR antibody can be used for detection of PIR by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PIR
NCBI Official Name
Pirin (iron-binding nuclear protein)
NCBI Organism
Homo sapiens
Background Reference 01
Adams, M. (2005) J. Biol. Chem. 280 (31), 28675-28682.
Other Product Names
PIR
Tested Applications
ELISA, WB, IHC
Protein ID
4505823
Physical Properties
Liquid
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