PIR Antibody

PIR is a member of the cupin superfamily. The protein is an Fe (II) -containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication.This gene encodes a member of the cupin superfamily. The encoded protein is an Fe (II) -containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PIR antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

PIR antibody can be used for detection of PIR by ELISA at 1:312500. PIR antibody can be used for detection of PIR by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.