TBX5 Antibody

Product Specifications

Background

TBX5 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX5 is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in TBX5 have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for TBX5.

NCBI Gene ID

6910

Swiss Prot

Q99593

Accession Number

NP_000183

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Zebrafish

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBX5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TBX5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

58 kDa, 60 kDa, 53 kDa, 39 kDa, 58 kDa

Applications Notes

TBX5 antibody can be used for detection of TBX5 by ELISA at 1:62500. TBX5 antibody can be used for detection of TBX5 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.